As it turns to the northeast, it spends much of its time running along the east side of the CSX Brooker Subdivision.

SR 235 then intersects U.S. Route 441 and subsequently curves off to the east as it breaks away from CR 241, which continues straight north.

As it leaves Alachua, it enters the site of the former town of Newnansville at Newmansville Cemetery, an area which has been on the National Register of Historic Places since 1974.

SR 235 continues to the east briefly leaving the LaCrosse city limits where it crosses a bridge over Rocky Creek, then enters and leaves the city again, maintaining that east-west trajectory until it intersects what would seem to be the northern terminus of CR 231, which secretly continues in a multiplex with the route.

Before SR 100 enters Union County, though, CR 235 turns right onto a street known as the "Lester Johns Memorial Highway."

County Road 235A is a suffixed alternate of CR 235 that spans from southwestern Alachua to the ghost town of Traxler.

The northeast corner of US 441 and CR 235A is primarily dominated by the grounds of Santa Fe High School.