State Road 25A is an unsigned designation for part of U.S. Route 441 along Marion Street in Lake City, Florida.

The road runs in a straight northwest to southeast trajectory until it curves to the southwest and terminates at US 41/SR 25/SR 100 just south of the interchange with I-10.

The first segment is entirely in Leesburg along the eastern shores of Lake Denham on the west side of U.S. Route 27 and Singletary Park beginning one mile north of the northern terminus of CR 33, and ending 0.7 miles south of the intersection with State Road 44.

Like the one further south, it runs along the west side of the parent route but this segment enters Fruitland Park.

The northern terminus is at US 27-441 in Fruitland Park just south of the intersection with Cook Street and Register Road.

CRs 25A/234 enters a portion of Paynes Prairie State Preserve where it terminates with US 441/SR 25, as CR 234 briefly turns east to join southbound US 441 until it reaches Northeast First Street.

Eventually it enters Alachua entirely passing south of a University of Florida Dairy Research Unit.

It begins as Northwest 174th Avenue at US 27/41, which runs east and west until it reaches Southeast 17th Street, where it turns north.