SR 329 traveled north as a four-lane divided highway through a mostly wooded area.

The state road ended at CR 232 (NW 53rd Avenue) at an unsignalized T-intersection.

[3] Since the transfer of the southern portion of SR 329 to the city and county, many changes were constructed along the road.

[7] FDOT and Alachua County made an agreement for transfer of South Main Street in March 2005, but did not consummate the transfer until April 2012 (north of Depot Avenue) and June 2013 (south of Depot Avenue).

Subsequently, in August 2013, the county transferred the portion north of Southwest 16th Avenue (SR 24A/SR 226) to the city of Gainesville.