Flow-FISH

[1][2][3] Flow-FISH is most commonly used to quantify the length of telomeres, which are stretches of repetitious DNA (hexameric TTAGGG repeats) at the distal ends of chromosomes[4] in human white blood cells, and a semi-automated method for doing so was published in Nature Protocols.

This type of Flow-FISH has been used to study latent infection of viruses such as HIV-1 and EBV,[8][9] but also to track single cell gene expression and translation into protein.

Flow-FISH thus allows for a higher throughput analysis of telomere length in blood leukocytes, which are a readily available form of human tissue sample.

Because cow thymocytes take up LDS751 dye to a lesser extent than their human counterparts, they may be reliably differentiated via plotting and gating the desired populations.

Other cell types that have not in the past proven to be good candidates for flow-FISH can be analyzed via extraction of nuclei and performance of the technique on them directly.

DNA is denatured via heat and formamide treatment, PNA probe is allowed to hybridize, excess probe is washed away, reading is taken in flow cytometer
Multiparametric analysis allows for differentiation of cell types within one sample, allowing for internal control, and analysis of leukocyte subtypes.