Sodium fluorescein is added into the systemic circulation, the retina is illuminated with blue-green light at a wavelength of 490 nanometers, and an angiogram is obtained by photographing the fluorescent green light that is emitted by the dye.

[3] Fluorescein angiography was pioneered by German ophthalmologist Achim Wessing, who published his findings in 1969. times are approximate Fluorescein enters the ocular circulation from the internal carotid artery via the ophthalmic artery.

This accounts for the short delay between the "choroidal flush" and retinal filling.

Causes of hyperfluorescence: Causes of hypofluorescence: Fluorescein angiography is used by physicians specializing in the treatment of eye diseases (ophthalmologists) to evaluate the vasculature of the retina, choroid, optic disc, and iris.

[3] Among the common groups of ophthalmologic disease, fluorescein angiography can detect diabetic retinopathy (neovascularization), vein occlusions, retinal artery occlusions,[4] edema of the optic disc, and tumors.