It was located in the Fordham section of the Bronx on Valentine Avenue near Kingsbridge Road, which at the time was a relatively undeveloped area, and inconvenient for patients and their families.

On May 11, 1907, the hospital moved to a 4-acre (1.6 ha) location just northwest of the intersection of Southern Boulevard and Crotona Avenue, adjacent to what was then St. John's College, now Fordham University.

[4] Medical students from the Fordham University School of Medicine (1905–1921) interned at the hospital.

[5] In the mid-1930s, the Federal Art Project sponsored interior murals by artists Emily Newton Barto and Elizabeth Deering.

Amongst those opposing the closure were Bronx Community Board 6 and Borough President Robert Abrams.