[1] It is the referral hospital for the districts of Bundibugyo, Kabarole, Kamwenge, Kasese, Ntoroko and Kyenjojo.

[2][3] Fort Portal Hospital lies within the city of Fort Portal, approximately 148 kilometres (92 mi), by road, west of Mubende Regional Referral Hospital.

[4] This location is approximately 294 kilometres (183 mi), west of Mulago National Referral Hospital, in Kampala, Uganda's capital and largest city.

[7] The hospital is designated as one of the 15 "Internship Hospitals" where graduates of Ugandan medical schools can serve one year of internship under the supervision of qualified specialists and consultants.

The Olympic rower and medical missionary, Charles Sergel, worked at Fort Portal Hospital between 1938 and 1939.