In the spring of 1896, he made his first trip to the African interior to explore the botany and people in the northern part of the Congo Basin.
He was a scientist of independent means who explored Europe and North Africa and in 1891, at age 28, privately published a key to the families of flowering plants of the world.
A second edition of his key appeared in 1917 and was based on Engler & Prantl's Die Natürlichen Pflanzenfamilien and Das Pflanzenreich.
Thonner's German plant key of 1891 was translated into English for the second time in 1981, and became more accessible to English-speaking botanists, rivalling that of John Hutchinson and Families of Angiosperms by Bertel Hansen & Knud Rahn[2] (1969).
Thonner also used back-up features ensuring correct choice of the family even when there is uncertainty about classifying botanical structures.