Fura-2, an aminopolycarboxylic acid, is a ratiometric fluorescent dye which binds to free intracellular calcium.

[1] It was the first widely used dye for calcium imaging, and remains very popular.

The use of the ratio automatically cancels out confounding variables, such as variable dye concentration and cell thickness, making Fura-2 one of the most appreciated tools to quantify calcium levels.

The high photon yield of fura-2 allowed the first real time (video rate) measurements of calcium inside living cells in 1986.

[2] More recently, genetically encoded calcium indicators based on spectral variants of the green fluorescent protein, such as Cameleons,[3] have supplemented the use of Fura-2 and other small molecule dyes for calcium imaging, but Fura-2 remains faster.