In the following years the network was further expanded, including a connection from Güstrow to Neubrandenburg opened in 1864, which was extended to Stettin (now Szczecin in Poland) in 1867.

From 1895, trains from Berlin to Rostock and Copenhagen ran via Güstrow instead of on the direct route via Plaaz on the Lloyd Railway.

After the Second World War, the Lloyd Railway south of Plaaz was dismantled as reparations to the Soviet Union.

Even after the reconstruction of the Lloyd Railway in 1960, the route was particularly important for long-distance services between Rostock and Berlin with more than half of the Durchgangszug (express) trains continued to run via Güstrow.

In subsequent years, the line speed was raised to 120 km/h and the last crossing opportunity located in former Mistorf station was removed.