Gainesville is a city in Ozark County, Missouri, United States.

According to the United States Census Bureau, the city has a total area of 2.87 square miles (7.43 km2), all land.

There were 406 housing units at an average density of 141.5 per square mile (54.6/km2).

40.4% of all households were made up of individuals, and 20.8% had someone living alone who was 65 years of age or older.

37.8% of all households were made up of individuals, and 20.4% had someone living alone who was 65 years of age or older.

The school district covers 339 square miles and serves several rural communities including Thornfield, Wasola, Tecumseh, and Pontiac.

The Gainesville R-V School District is "fully accredited" and has A+ designation.

Gainesville High School is unranked by U.S. News & World Report and has a graduation rate below the state median.