Part of the Depot was built around 1860 to serve the Florida Railroad, which reached Gainesville from Fernandina in 1859.

[2] It is one of only three surviving railroad depots in the state built prior to the start of the American Civil War.

[6][7] Depot operations were moved to a new building where East University Avenue crossed the rail line in January, 1948.

[10] The depot has been restored, and painted in the same colors, and with the same style metal roof shingles, used in 1910.

This article about a property in Alachua County, Florida on the National Register of Historic Places is a stub.