Gainsborough Creek is a tributary of the Souris River in south-eastern Saskatchewan and south-western Manitoba.

In 2014, extended heavy rainfall in the south-eastern Saskatchewan and south-western Manitoba resulted in severe flash flooding across much of the region.

[8][9] The source of the Gainsborough Creek is between Wawota and Fairlight, Saskatchewan, just north of Highway 48.

It continues southward until it comes up to Highway 18 and the village of Gainsborough at which point it starts to head east into Manitoba.

After crossing the Road 251 south of Pierson, it heads north-east where it joins up with the Souris River just downstream from where the Antler River meets the Souris, near the junction of Highway 83 and Highway 3.