Gastrotheca zeugocystis is a species of frogs in the family Hemiphractidae.

It is endemic to Peru and only known from its type locality on the Cordillera de Carpish, Huánuco Region.

[1][3] The specific name zeugocystis refers to the paired brood pouches in this species (from the Greek zeugos, meaning pair, and the Greek kystis, meaning sac).

[2] Gastrotheca zeugocystis inhabits cloud forest at about 2,920 m (9,580 ft) above sea level.

It is threatened by deforestation caused by agricultural expansion and firewood extraction.