It connects the Florida state line with Higgston, via Lakeland, Willacoochee, Douglas, and Hazlehurst.

It travels concurrently with U.S. Route 221 for extensive distances, a short piece in Lakeland and a longer one from Douglas to Uvalda.

SR 135 begins at the Florida state line intersecting with County Road 141 (CR 141) south.

[6] SR 135 enters Coffee County south of Douglas, where it also travels parallel to a former Central of Georgia Railway right-of-way.

It begins at an intersection with SR 135 south of Darsey Pond, then curves to the northeast.

At Union Church and the cemetery across the street from it, the road begins to curve to the northwest and then travels to the west just south of the Lakeland city limits.

Burnt Church Road continues to the west as an unnumbered street leading to US 221.

It begins at an intersection with the SR 135 mainline just south of the Lakeland city limits, then intersects SR 135 Loop (only signed as Burnt Church Road) before entering the city.

intersects US 129/US 221/SR 11/SR 31/SR 37/SR 122 (Main Street) and travels concurrently with them for two blocks, then turns north onto North College Street in a one-block concurrency with SR 11 Byp., which turns left at East Church Avenue.

It travels to the southeast on Jefferson Street, concurrent with US 221 Truck and SR 135 Conn., which both begin at this intersection, as well.

The three highways cross over some railroad tracks of Norfolk Southern Railway (NS) and then leave the city limits.

They cross over some railroad tracks of NS Railway and then intersect US 341/SR 27 (Golden Isles Parkway).