SR 241 begins at the Florida state line in southern Decatur County, where the roadway continues as County Road 65 (Attapulgus Highway).

It heads east-northeast to an intersection with SR 309 (Fowlstown Road).

Then, the road curves to the northeast, crossing over Little Attapulgus Creek and a CSX Transportation railroad line.

It then heads north-northeast, until it enters Attapulgus, where it meets its northern terminus, an intersection with US 27 Business/SR 1 Business.

[1] No section of SR 241 is part of the National Highway System, a system of routes determined to be the most important for the nation's economy, mobility and defense.