On 11 January 2018, Antzoulas made his professional debut in Greek Cup against Atromitos, while displaying his obvious talent in the U17 and U19 Super Leagues.
On 28 July 2018, Antzoulas was loaned to Fiorentina, with a purchase option of €2 million for the summer of 2019.
He has enjoyed a successful season with Fiorentina, winning the Coppa Italia Primavera and receiving a call-up to the senior squad, for three Serie A matches against Napoli in February and Milan, Parma in May.
Interestingly, in the event Antzoulas made an appearance for the Fiorentina first team, his release clause of €2 million from Asteras Tripolis would be automatically activated.
[4] After spending two seasons with Újpest in Hungary, on 18 July 2024, Antzoulas signed with HJK Helsinki in Finnish Veikkausliiga on a deal until the end of 2025.