This pattern of disulfide bridges between cysteine amino acids creates a motif called an inhibitory cystine knot found in various other spider toxins.

[1] Proteins with similar sequences to GiTx1 such as PaTx1 and PaTx 2, which are both phrixotoxins, have been shown to influence voltage-gated potassium channels in cardiac cells.

With a lower concentration (0.1-0.8 μg), the mice suffer from multiple symptoms such as rotating movements, disorientation and paralysis.

At higher concentrations (0.8-1.6 μg), other symptoms occur on top of paralysis with severe mobility difficulty, including grunting and cyanosis.

[1] The Toxicity of Grammostola iheringi's venom shows an LD50 of 0.20 μg/fly in Drosophila melanogaster while the LD50 of solely GiTx1 is slightly higher, namely 20.9 μg/g.