Giovanni Battista Acquaviva (1513-1569) was a Roman Catholic prelate who served as Bishop of Nardò (1536–1569).
On 22 May 1536, he was appointed by Pope Paul III as administrator of the Diocese of Nardò, [3] with the right to become bishop at age twenty-seven.
In 1563 he called a diocesan synod, which he solemnly celebrated in the presence of the chapter and the clergy of the city and diocese.
In 1568 Bishop Acquaviva brought the Carmelite monks to settled in the city.
He served as Bishop of Nardò until his death at the age of fifty-six on August 13, 1569.