Giovanni Nepomucene, Principe Ruspoli (June 28, 1807 – November 6, 1876) was the 5th Principe di Cerveteri, 5th Marchese di Riano, 10th Conte di Vignanello, and Prince of the Holy Roman Empire.

He was the son of Alessandro Ruspoli, 4th Prince of Cerveteri and his wife Mariana Gräfin Esterházy de Galántha and an ancestor of the Line I of the Principi Ruspoli.

He married in Rome, May 16, 1832, Barbara dei Principi Massimo (Rome, December 20, 1813 – Rome, November 1, 1849), by whom he had four children: