Giovanni Vincenzo Acquaviva d'Aragona (born between 1490 and 1495 in Naples in Italy, died 16 August 1546 in Itri) was a Cardinal of the Roman Catholic Church.
Belonging to an illustrious and powerful noble family from the south, Giovanni Vincenzo Acquaviva d'Aragona was born in Naples, the son of Andrea Matteo III Acquaviva d'Aragona, eighth duke of Atri, 15th Count of Conversano and Count of Caserta, and his wife Isabella Piccolomini.
His nephew, Claudio Acquaviva, son of his brother Giannantonio Donato, 9th Duke of Atri, was the fifth General of the Jesuits.
In February 1537, he was elected bishop of the diocese of Melfi and Rapolla; and in June that year, Archpriest of Santa Maria in Platea di San Flaviano.
Pope Pius V sent him as ambassador to Phillip II of Spain in an attempt to resolve a question of ecclesiastical jurisdiction.