He traveled to Rome where he admired the fresco painted in 1614 by Guido Reni, depicting the Aurora.
In 1615 he painted two ceiling canvases of Putti Supporting the profile of Michelangelo for the Casa Buonarroti and in the same period frescoed a choir of musician-angels for the dome of the church of the Ognissanti in Florence.
His masterpiece is said to be frescoes in the chapel of Saint Catherine inside the Palazzo Rospigliosi-Pallavicini in Pistoia.
On the left internal wall of the chorus of the church of San Bartolomeo in Cutigliano, is a canvas of Mannozzi's the Circumcision (1620).
He painted a fresco cycle on the vault of the Mellini Chapel in Santa Maria del Popolo which displays The Story of Saint Nicholas of Tolentino in 1623–24.