He had been attempting to rescue injured soldiers lying in the open between the two sides, an action for which he received Italy's Silver Medal of Military Valor.
[5] While at Bari, he conducted his own experiments confirming the usefulness of the lepromin skin test recently devised by Kensuke Mitsuda,[6][7][8] introducing it to Italian medicine.
On his own, Mariani worked on venereal diseases, developing a rapid method of identifying cases of syphilis microscopically to improve efficiency while performing tens of thousands of treatments, mostly using bismuth and arsenobenzols.
His work sought to integrate the findings of histopathological surveys into the existing diagnostic methodology based on morphological analysis[1] and also to pay greater attention to subcutaneous diagnosis and treatment.
He continued to research a number of areas[2] including dermatological diagnosis and treatment in oncology,[9] but increasingly focused on allergology.