Hartpury College already had a women's team competing in the BUCS rugby union leagues.

[12] Ahead of the 2022-23 Premier 15s season, Gloucester Rugby announced a significant increase in funding to Gloucester-Hartpury by almost 500%, matching an enhanced contribution from Hartpury University.

[13][14] Former Gloucester player and Gloucester-Hartpury coach James Forrester was appointed CEO of the team in 2023.

[22] The Gloucester-Hartpury squad for the 2024–25 season is:[23] Note: Flags indicate national union under World Rugby eligibility rules.

Notes Gold background denotes championsSilver background denotes runners-up The following table details the past performance of Gloucester-Hartpury against different opponents in Premiership Women's Rugby, between the start of the 2017-18 season and the end of the 2023–24 season.

The following are players who have represented their countries at the Rugby World Cup whilst playing for Gloucester-Hartpury: