In 2017, they were selected as one of the franchises for the new Premier 15s league, but they also cater for new, aspiring and social players within their 3rd XV squad.

[7] In 2016, Harlequins announced a partnership with Women's Premiership club Aylesford Bulls Ladies.

In 2017, Harlequins Ladies were awarded a franchise for the new Women's Super League, later renamed Premier 15s.

On 30 August 2018, it was announced that the Harlequins Women 3rd XV squad would be playing their home matches at the brand new facilities at Grasshoppers RFC.

[18] The Harlequins Women squad for the 2021–22 season is:[19] Note: Flags indicate national union under World Rugby eligibility rules.