Created and executive produced by Chris Gifford and Valerie Walsh Valdes, the series is a spin-off of the animated television series Dora the Explorer and follows Dora's cousin Diego, an 8-year-old boy whose adventures often involve rescuing animals and protecting the environment.

Supporting characters include Diego's sisters Alicia and Daisy, his companion Baby Jaguar, Click, a camera that locates animals, and Rescue Pack, a messenger bag Diego wears that can transform into any object.

Whenever a carnivorous predator is featured on the show, its diet is not mentioned, unlike with when herbivores are shown.

In the United States, Go, Diego, Go aired on CBS, Nickelodeon, and the Nick Jr. Channel.

The show also aired in the United States on Spanish television network Univision on its Planeta U Saturday morning children's block from its debut on April 5, 2008 to May 24, 2014.

A touring live version of the series was staged in 2007, starring Richard J. Portela as the role of Diego.