The Pisa–Livorno–Rome railway is a double track main line connecting Pisa with the Tyrrhenian Sea and Rome.

Grosseto railway station is situated in Piazza Guglielmo Marconi, near the centre of the city, just north of the old town.

Piazza Marconi, often erroneously referred to as Station Square, is known for its statue of a buttero (1953) by sculptor Tolomeo Faccendi.

At the ground floor level, the passenger building's central facade is characterized by five portals with architraves topped by round arches.

The latter line is currently used by historic Treno Natura trains, which also operate excursions to local castles around Montalcino.

The station is the terminus of many urban and suburban bus lines operated by Autolinee Toscane, the transport company serving the whole of Tuscany.