Rio 2016 Olympian José Ramos accepted a continental berth from the Americas as the nation's top-ranked judoka outside of direct qualifying position in the IJF World Ranking List of June 28, 2021.

Rio 2016 Olympian Charles Fernández secured a selection in men's event with a gold medal victory at the 2019 Pan American Games in Lima.

[11] Guatemala qualified one boat in the women's lightweight double sculls for the Games by finishing last in the A-final and securing the second of three berths available at the 2021 FISA Americas Olympic Qualification Regatta in Rio de Janeiro, Brazil, signifying the country's return to the sport for the first time since 1984.

[13] M = Medal race; EL = Eliminated – did not advance into the medal race Guatemalan shooters achieved quota places for the following events by virtue of their best finishes at the 2018 ISSF World Championships, the 2019 ISSF World Cup series, the 2019 Pan American Games, and Championships of the Americas, as long as they obtained a minimum qualifying score (MQS) by May 31, 2020.

Scarleth Ucelo accepted a spare berth unused by Oceania as the next highest-ranked weightlifter vying for qualification in the women's +87 kg category based on the IWF Absolute World Rankings.