GO Transit buses and trains on the Kitchener corridor and Via Rail also stop at Guelph Central Station.

The initial route of the GRC was south along Woolwich Street, through the downtown and along Dundas Road, with a second line running from the Sleeman owned Silvercreek Brewery on Waterloo Avenue, to the Canadian Pacific and Grand Trunk (later Canadian National) Railway stations.

The carbarn later served as the garage for the Guelph Transportation Commission buses until the 1970s, and still stands today at 371 Waterloo Avenue.

Sleeman operated a brewery on Waterloo Avenue and expected that his employees would travel back and forth to work on his system.

In 1903, the city of Guelph purchased the street railway for $78,000, which included eight miles of track, eight closed and three open cars.

Freight service had been introduced in 1900 using a small four-wheel locomotive, with traffic being interchanged with the Grand Trunk Railway.

This business increased to a point where in 1911 a new 27-short ton locomotive, #26, was purchased from Preston Car & Coach, along with two 2-truck 'Prairie' type streetcars, #60 and #70.

A second freight interchange was added on Suffolk Street in 1915 and a connection was made with the new Toronto Suburban Railway line in 1917.

The Suffolk line was removed in 1929 due to its poor condition and the cost of rebuilding, being replaced by bus service.

Guelph Transit's garage and other city works moved from Waterloo Avenue to 12 Municipal Street in the 1970s.

[12] Global positioning system (GPS) technology and advanced computer modelling allow riders to receive accurate, real-time arrival and departure information via the Internet, handheld devices (including Palms, Blackberries, and Web-capable cellular phones), or their telephones.

[13] The new system features web-based map displays with local streets and routes, and real-time information available on the web.

Guelph Transit added holiday service in 2007 as well as additional routes serving the south end of the city.

[14] As identified in the 2010 budget, Guelph Transit is modifying frequency and service hours in order to achieve targeted savings.

In January 2020, The City of Guelph announced that it will be investing $177 million to purchase 67 electric transit busses and build a new bus storage facility.

Work was underway to convert the Guelph Central Station and current Greyhound Bus Terminal into a Regional Transit Facility by 2014.

Each weekday, more than 5,000 passengers board Guelph Transit, to travel on one of the 15 different routes that operate out of the bus bays adjacent to the train station.

This service is provided from September to April from Tuesday to Sunday, with the exception of winter break, reading week and statutory holidays.

[20] There is also a Downtown-University Bus service where students can board at either University Centre or Guelph Central Station.