Don Guido Colonna dei principi di Paliano (16 April 1908 – 27 January 1982) was an Italian diplomat and European Commissioner.

He was the seventh child, and fourth son, of Don Stefano Colonna, dei principi di Paliano (1870–1948) and Maria Dorotea Cianciulli (1875–1942).

The diplomat and Italian ambassador to London during the First World War Marquess Guglielmo Imperiali (1858–1944), was the husband of his paternal aunt.

After the end of his political career, Guido Colonna di Paliano returned to the private sector as President of the Italian department store chain "Rinascente".

[4] On 8 June 1938 in New York, Colonna, at the time Italian vice consul at Toronto, married Tatiana Conus, daughter of Julius Conus (Yúlij Eduárdovič Konyús), a Russian pianist and composer of French origin, and of Princess Maria Alexandrovna Lieven.