Guinea-Bissau competed at the 2020 Summer Olympics in Tokyo.

[2] It was the nation's seventh consecutive appearance at the Summer Olympics.

[3] Guinea-Bissau qualified one judoka for the women's half-lightweight category (52 kg).

Rio 2016 Olympian Taciana Cesar received an African continental quota as the nation's top-ranked judoka outside of direct qualifying position on the IJF World Ranking List of June 28, 2021.

[4] Guinea-Bissau qualified two male freestyle wrestlers, both of whom progressed to the top two of their respective events at the 2021 African & Oceania Qualification Tournament in Hammamet, Tunisia.