HMS Ambuscade was a 40-gun fifth rate frigate of the Royal Navy.
Embuscade was a one-off 38-gun design by Pierre Chaillé, with 26 × 8-pounder and 12 × 4-pounder guns and was launched at Le Havre on 19 March 1745.
She was captured in the English Channel by HMS Defiance on 21 April 1746.
Ambuscade fought at the First Battle of Cape Finisterre on 3 May 1747, commanded by Captain John Montagu.
She captured the privateer Vainqueen on 12 July 1757, and fought with Edward Boscawen against Jean-François de La Clue-Sabran at the Battle of Lagos on 19 August 1759.