Hamida Banu's mother was Maah Afroz Begum, who married Ali Akbar Jami in Paat, Sindh.

[9] Initially, Hamida refused to meet the emperor; eventually after forty days of pursuit and at the insistence of Dildar Begum, she agreed to marry him.

She refers to her initial reluctance in the Humayunama,[10] I shall marry someone; but he shall be a man whose collar my hand can touch, and not one whose skirt it does not reach.The marriage took place on a day chosen by the Emperor, an avid astrologer himself, employing his astrolabe, at mid-day on a Monday in September, 1541 (Jumada al-awwal 948 AH) at Patr (known as Paat, Dadu District of Sindh).

[2][11][12] The marriage became "politically beneficial" to Humayun, as he got help from the rival Shia groups during times of war.

First, in the beginning of the following December, she and her new born went into camp at Jūn, after traveling for ten or twelve days.

Then in 1543, she made the perilous journey from Sindh, which had Qandahar for its goal, but in course of which Humayun had to take hasty flight from Shal-mastan, "through a desert and waterless waste".

In 1544, at a camp at Sabzawar, 93 miles south of Herat, she gave birth to two daughters, who died on the return journey from Persia.

[18] Thereafter, she returned from Persia with the army given to Humayun by Shah of Iran, Tahmasp I, and at Kandahar met Dildar Begum, and her son, Mirza Hindal.