Harker Creek is a stream located in Iowa County, Wisconsin.

The upper end of the creek is approximately 10 km (6.2 mi) west-northwest of the county seat, Dodgeville.

The creek flows roughly north-south, and is approximately 7.5 km (4.7 mi) in total length.

It drains into Flint Creek at its northern end at 248 m (814 ft) above sea level.

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