She was sporadically tutored by Henning Tideman, the teacher of her brother Anders Nordenflycht (1710–1740), in Latin and German, and studied philosophy and theology as an autodidact, being otherwise only educated in domestics and accomplishments.

Johan Tideman and his naturalism and philosophy made a great impact upon her development and satisfied her intellectually, but she opposed their engagement because she did not find him physically attractive.

On 23 April 1741 she married Jacob Fabricius (1704–1741), who had been her French language teacher, and was appointed chaplain of the admiralty at Karlskrona prior to their marriage.

Before here departure to Karlskrona, she wrote the poetic collection Cronstedtska poemboken, which was a gift to her friend countess Margaretha Beata Cronstedt.

She retired to a rented cottage at Lidingö outside Stockholm to mourn, during which she wrote the Den sörgande turtur-dufvan, poems describing her longing for her lost love and the nature of sadness and loss.

In 1742, she debuted as a published author with Svenska fruntimrets klagan (The Lament of the Swedish Woman), a poem over queen Ulrika Eleonora.

[6] On 14 April 1753, Nordenflycht was inducted in Tankebyggarorden, a literary academy in Stockholm under the leadership of Carl Fredrik Eckleff, founded shortly before based on French role models, which had the purpose to reform the contemporary Swedish literature.

[11] Johan Fischerström, born in 1735, was a student from Lund University with radical ideas and literary interests, who had made her acquaintance in the circles of the Tankebyggarorden.

When Nordenflycht moved to Lugnet, she arranged for Fischerström to be given a position at the Sjöö Castle, the home of her personal friend Cathérine Charlotte De la Gardie.

With her poem Vigtiga frågor til en lärd, in which she asked Ludvig Holberg to bring order in the confusion between religion and science, she became known outside of Sweden.

[23] In parallel to being a supporter of the scientific Age of Enlightenment, however, she was somewhat skeptic to the idea that the knowledge of science (in opposition to the emotional mysticism of religion) in itself could give happiness, a doubt she expressed in Till Criton (1754), written to Carl Klingenberg.

[26] As a poet, Nordenflycht is known for describing the existential conflict between religion and science during the age of enlightenment, and for her depictions of the emotional symptoms of human love and sorrow.