[8] The zoo's mission includes four pillars, "Conservation, Research, Recreation, and Education" which are represented by the four squares in the logo.

In addition to the World Publishing Company, the zoo soon connected with another notable Omaha business: Union Pacific.

In 2011, the zoo began developing the land at the stadium to become the new parking area and visitor center, leaving a small memorial at the location of home plate.

[21][22] Species include: A new exhibit built on previously undeveloped space, the Asian Highlands area is a $22 million, 8-acre (3.2 ha) recreation of the highlands of central Asia, including India and China, designed to simulate a mountain ascent, featuring species native to the region as well as a Yeti Camp, acting as a hub for visitors with themed food and gift options, and a Kid's Discovery Trail.

The building features several exhibits: The Desert Dome opened in April 2002 at a cost of $31.5 million (includes Kingdoms of the Night).

[25] Beneath the Desert Dome is the Kingdoms of the Night, and both levels make up a combined total of 84,000 sq ft (1.9 acres; 7,800 m2).

[25] Expedition Madagascar opened May 7, 2010, and has many animals including lemurs, straw-coloured fruit bats, and giant jumping rats.

The building allows visitors to learn about Madagascar, an area considered one of the top hotspots for biodiversity because it is home to the largest number of endemic plant and animal species.

The first phase is the outdoor habitat that includes two 65-foot (20 m)-tall, 100-short-ton (91-metric-ton) Banyan trees interconnected with vines enclosed by a stainless steel netting.

The exhibit underwent extensive renovations in early 2021, closing briefly due to the COVID-19 pandemic, before reopening in July 2021.

The expansion includes an all-new indoor/outdoor siamang exhibit, an updated elevator building and plaza, a café, a walkway, and a seating area.

It features displays of aquatic habitats from polar regions, temperate oceans, the flooded Amazon rainforest, and coral reefs.

Other tanks include multiple species of jellyfish, a Giant pacific octopus, bonnet sharks, and open-ocean schooling fish.

Another addition is a touch tank which allows visitors to feel the textures of various starfish, shells, and possibly a chain catshark or one of its empty eggs.

The Kingdoms of the Night features a wet cave (with a 14 ft or 4.3 m deep aquarium), a canyon, an African diorama, a eucalyptus forest, a dry batcave, and a swamp.

[30] It is one of the largest indoor rainforest exhibits in the world; it occupies an 80-foot (24 m) tall building that spans 1.5 acres (0.61 ha) and is located just inside the main entrance.

The jungle has won several awards, including "Single best zoo exhibit in the country" in 1994 by the Family Life Magazine; "Significant Achievement Award for Exhibit Design" in 1993 by the American Association of Zoological Parks and Aquariums; "Top ten designs in the world" in 1992 by Time, and "Top eight US engineering accomplishments" in 1992 by the National Society of Professional Engineers[32] The Owen Sea Lion Shores opened on September 4, 2020, costing $27.5 million.

It features a 275,000-gallon saltwater pool with natural wave chambers, shallow beaches for young pups learning to swim, fish feeders that release fish and calamari at random times and places in the exhibit encouraging hunting behavior, a shaded seating area with a capacity of up to 170, and an underwater viewing cavern.

The structure is covered with 142,000 sq ft (13,200 m2) of two-inch nylon mesh that is supported by a system of cables and poles.

Situated inside the zoo's main entrance, the 21,000-square-foot (2,000 m2) Mutual of Omaha's Wild Kingdom Pavilion was completed in the spring of 1987.

The building currently houses reptiles, insects, amphibians, and small mammals, while also providing business offices, a 312-seat multimedia auditorium, and classrooms.

The area also features turf green space, art sculptures installed prior to the demolition of Bear Canyon, and a small event center.

The zoo also features Lozier IMAX Theater, Glacier Bay Landing, a Budgie Encounter in the Adventure Trails children's play area.

[35] Omaha's Henry Doorly Zoo and Aquarium is known worldwide for its work in the field of artificial insemination of large cats.

The mission of the behind-the-scenes area, which is not accessible to visitors, is to address wild amphibian decline by continually advancing conservation efforts through evolving welfare, reproduction, collaboration, and reintroduction.

The 3,800 square-foot facility features 13 temperature-controlled rooms, a specialized wastewater treatment system, insect culturing area and thorough disinfection protocols to ensure the entire space remains pathogen-free.

It goes over the African veldt (ostriches and giraffes), cheetahs, the railroad tracks, the Garden of the Senses, the koi lagoon, and the lions.

Major zoo educational programs include on-site preschool and high school courses, internships, and volunteer work.

Other programs include field trips, guided tours, educator workshops, and ambassador animal presentations.

Other educational programming includes overnight campouts, scouting events, summer camps, birthday parties, and on-site speakers.