Southeast of HaShiv'a Interchange the road crosses Beit Dagan Junction with Route 412, which connects it with Rishon Letzion and Yehud.

The road then passes the Volkani Institute, the Tzrifin Military base, Assaf HaRofeh Medical Center and various industrial parks.

After Nachshon Junction, it begins a slow ascent into the foothills of the Judean Mountains, passing several more agricultural communities and national forests.

After the establishment of the State, this road operated for many years as one of the main routes connecting the coastal plain with Jerusalem via Highway 38 and Sha'ar HaGai (Bab al-Wad).

The Israel Ministry of Transport has announced a grade separation of Highway 44 with the Lod-Na'an Railway line at HaHashmonain Street adjacent to Ramla North Junction.