They have 3 dorsal lines that can be yellow, tan, green, or brown that are used to most easily identify the species.

[3] The Osteopilus genus tend to follow a generalist diet and usually choose prey based on their size [4] Osteopilus pulchrilineatus is one of four endemic species to Hispaniola and can only survive in a specific set of environmental conditions.

Some of these include residential development, mining, invasive species and diseases, habitat loss due to ranching and wood logging.

[7] Habitat loss for agriculture, usually for the production of sugarcane, is another huge threat to tree frogs in the region.

[5] Another threat to their populations comes from the decline in stream quality in Hispaniola from these human activities.