The Holtwood Arboretum over 5,000 acres (20 km2), also known as Holtwood Preserve, is a recreation area, arboretum, and wildflower preserve located on New Village Road off Pennsylvania Route 372 in Lancaster County, Holtwood, Pennsylvania.
The site is owned by PPL Corporation and provides public recreation, camping, hiking, picnicking, sightseeing, fishing, and hunting.
The lower Susquehanna River runs through the preserve, which also contains substantial fish ladders (said to be the largest in the United States) and a museum of Native American artifacts.
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