It was founded in 1948 by the Medical Mission Sisters, a Roman Catholic religious order for women based in Philadelphia, USA.

In that year the diocese was no longer able to bear the financial burden of running the hospital and handed it over to the Government of Punjab.

The Medical Mission Sisters went to work and focus exclusively at the Holy Family Hospital, Karachi.

This hospital is accredited with the College of Physicians and Surgeons Pakistan to train doctors for specialization in medicine, surgery and obstetrics.

This is recognition of the high quality care provided and the important role played by the hospital over the years.