The Church of the Holy Trinity is an Episcopal parish church located at 316 East 88th Street between First and Second Avenues in the Yorkville neighborhood of Manhattan, New York City.

The parish was originally located on the northeast corner of Madison Avenue and East 42nd Street in a Victorian cottage ornéé (ornamental cottage) designed by Jacob Wrey Mould.

"[2] The engagement by the parish of E. Walpole Warren as rector and pastor in September 1887 was the subject of the notable United States Supreme Court case Church of the Holy Trinity v. United States.

A mission church was built on this land from 1895 to 1899, designed by Barney and Chapman.

[6] Although the mission was administered by St. James, it was called Holy Trinity.