She attended Kabale High School' for her O-Level studies, And Trinity College Nabbingo for her A-Level education.
Hope Mwesigye worked as a Senior State Attorney in the Ugandan Ministry of Justice for eight years prior to 1988.
Between 1988 and 1991, she worked as the Program Officer at Uganda Gender Resources Center, a private non profit organization.
She has also served as a Program Officer for FIDA (Uganda), another Ugandan non-profit specializing in championing the rights of women.
She lost her re-election bid in 2011 to Ronah Ninsiima, an Independent politician, who is the incumbent MP for Kabale Women's Representative.