[2] HSI Australia works on national and international conservation and animal protection policy and law, with a special responsibility to reach out to its regional neighbors.

A few years later, HSI Australia became a regional non-governmental organization with a team of staff & consultants, members across the country, and a budget of $2.5 million.

It runs numerous conservation and animal protection campaigns that cover a broad range in both marine and terrestrial areas.

The organisation is opposed to the export of live animals from Australia,[4] and believes that the frozen meat market is a readily available and ethical option for the industry.

The organisation works with the Australian Competition & Consumer Commission regarding legislation for pork and poultry, which includes their truthful labeling campaign.

Following the announcement of Japan's "Newrep-A" plan, scheduled to commence in December 2015, senior program officer Alexia Wellbelove stated: "It is now time that Japan listened to international public opinion and consigned their so-called research program and plans to resume commercial whaling to history where it belongs.

HSI Australia also campaigned alongside its international counterparts to ban the Canadian seal hunt, and is pushing for an end to the trade.

The group also campaigns against Australian Greyhound racing, with a focus placed upon the New South Wales (NSW) sector of the industry.

HSI Australia seeks greater transparency in the industry, including stricter welfare standards, and better reporting and investigation of cruelty and mistreatment.