A music video for "Hustler's Ambition" was directed by Anthony Mandler, and shows 50 Cent performing the song inside a warehouse whilst preparing for a boxing match.

[7] After opening with a sample of the Frankie Beverly and Maze song "I Need You",[4] the instrumentation provided by B-Money "B$ "is built around a "solid bassline", and features background vocals that harmonize with 50 Cent's voice.

[8] 50 Cent's lyrics muse "exactly how far he wants to go" in the entertainment business following his rapid rise to fame over the preceding two years, as well as his believed superiority to other contemporary artists: "Look at me, this is the life I chose / Niggaz around me so cold, man my heart done froze...

[5] Writing for The Michigan Daily, Andrew Kahn noted the "old-school, pre-fame hunger" present in "Hustler's Ambition" due to the use of the "I Need You" sample.

[6] Steve Juon of RapReviews commented on 50 Cent's enunciation on the song, observing how the background vocals "never distract from 50's words" and that his "skills as an orator helped him rise to his current station in life".

[6] In his review for the Get Rich or Die Tryin' soundtrack for PopMatters, Mike Schiller observed that "Hustler's Ambition" effectively represents the struggles that 50 Cent's character faces in the corresponding film, pointing out that it emphasizes his "hunger for money" and also noted the song to be a "subtle shift in perspective" of 50 Cent's music, in that it had moved from "club bangers that serve no particular purpose other than to get people dancing" to "raps... spit through the voice of a fictionalized version of his own younger self".

[26] It opens with an off-camera monologue from 50 Cent, explaining the hardships often faced by those in poorer communities, whilst – on-camera this time – he enters a warehouse, passing two men as he arrives, containing several pieces of recording equipment.

Shots of 50 Cent travelling around the warehouse are then interspersed with those of him performing "Hustler's Ambition" into a microphone: after arriving in one room, he is shown to be repeatedly hitting a punching bag and lifting weights, as he trains for an upcoming boxing match.

Other members of G-Unit Records, including singer Olivia, are then shown to be present in the warehouse as 50 Cent continues to perform the song.