IMOD is an open-source, cross-platform suite of modeling, display and image processing programs used for 3D reconstruction and modeling of microscopy images with a special emphasis on electron microscopy data.

IMOD has been used across a range of scales from macromolecule structures[1] to organelles[2][3][4] to whole cells[5] and can also be used for optical sections.

IMOD was developed at the Boulder Laboratory for 3-D Electron Microscopy of Cells.

IMOD includes over 180 command line programs listed here and three main GUI programs: Image Format: The main image format supported by IMOD is MRC file format, which typically have a ".st", ".mrc" or ".rec" extensions and represent various types of "image stacks" which together might represent a tilt series or 3D volume.

Vector Format: IMOD saves and opens vector data in the form of contour (polygons) and meshes in an IMOD binary file format, typically with a ".mod" or ".fid" extension.