He probably began to practise as an apothecary as a youth; and owned the then famous Farmacia del Moro near the Cathedral.

He later took a leading role in the establishment of the more famous Accademia della Crusca, which published his Vocabulario of words accepted as the purest Italian.

His Umidi brethren expelled him for a time, because of his ruthless criticism of the Arameans, a party of academicians who maintained that the Florentine language was derived from Hebrew, Aramaic, or some other branch of the Semitic.

He also edited the works of Francesco Berni, and collected Tutti I trionfi, larri, mascherate o canti carnascialeschi, andati per Firenze dal tempo del Magnifico Lorenzo vecchio de 'Medici fino all'anno 1559 (the latter influenced Goethe's "Faust").

In 1868 Adamo Rossi published in his Ricerche per le biblioteche di Perugia three novelle by Grazzini, from a manuscript of the 16th century in the Comunale of Perugia; and in 1870 a small collection of those poems which have been left unpublished by previous editors appeared at Poggibonsi, Alcune poesie inedite.