Immunosequencing

Immunosequencing, sometimes referred to as repertoire sequencing or Rep-Seq, is a method for analyzing the genetic makeup of an individual's immune system.

Through V(D)J recombination a number of organisms take a relatively small number of genes coding for antibodies and T-cell receptors (TCRs) and produce a huge diversity of slightly different antibodies and TCRs.

As an immune system reacts to infections and other events, the number of different antibodies and TCRs it contains changes.

Immunosequencing is a technique utilizing multiplex polymerase chain reaction that allows for the sequencing and quantification of the large diversity of antibody and TCR genes composing an individual's immune repertoire.

[1][2] Immunosequencing in its modern context started being discussed in scientific literature in the early 2010s with the advent of more powerful gene sequencing techniques.