In silico PCR

Other information provided by in silico PCR tools may include determining primer location, orientation, length of each amplicon, simulation of electrophoretic mobility, identification of open reading frames, and links to other web resources.

[7][8][9] Many software packages are available offering differing balances of feature set, ease of use, efficiency, and cost.

[10][11][12][13][14] Primer-BLAST is widely used, and freely accessible from the National Center for Biotechnology Information (NCBI) website.

Dicey[15] is free software that outputs in-silico PCR products from primer sets provided in a FASTA file.

VPCR[3] runs a dynamic simulation of multiplex PCR, allowing for an estimate of quantitative competition effects between multiple amplicons in one reaction.

In silico PCR example result with FastPCR [ 7 ] [ 8 ] software.