Running for about 1.5 miles (2.4 km) in a general east–west direction, connecting Jasper-Pulaski Fish and Wildlife Area to U.S. Route 421 (US 421).

SR 143 begins at a four-way intersection with County Road 1650 West crossing north–south and County Road 550 North continues west from the western end of SR 143.

The wooded area becomes farmland before SR 143 has a four-way intersection with US 421 about midway between Medaryville and San Pierre.

[1][2][3] The highest traffic count for SR 143 in 2014 was at its western end, where 295 vehicles travel the highway on average each day.

The lowest traffic count during 2014 was at the eastern end of SR 143, where 264 vehicles travel the highway on average each day.