Towns include Uniontown, Paris Crossing, Canaan, and East Enterprise.

SR 250 is split in two north of Madison due to the Big Oaks National Wildlife Refuge (formerly the Jefferson Proving Ground).

SR 250 heads southeast from the western terminus in Brownstown towards State Road 39.

SR 250 heads east from I-65 and then has a concurrency with State Road 3.

Then eastern terminus of this section is an intersection with State Road 7.